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A. Suneetha ¹, Pandit M. Risheela ¹, R. Sujatha ¹

Affiliations
1 Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur- 522 002, Andhra Pradesh, IN

Abstract

The present method describes the development of a validated RP-HPLC for determination of ormeloxifene hydrochloride in presence of its degradation products or other pharmaceutical excipients. The drug substance was subjected to stress conditions of acid (2N HCl), alkali (2N NaOH), oxidative (30%H₂O₂), neutral, thermal and photolytic studies. Seperation was carried out on Agilent XDB C18 column,(250×4.6mm I.D, Particle size 5μm) by using mobile phase consisting of a mixture of buffer: acetonitrile: methanol (20:35:45,v/v/v) with the flow rate of 1.0mL/min. UV detection was performed at 245nm and retention time was found to be 5.48min. The method was validated for linearity, accuracy and precision. Linearity for ormeloxifene was in the range of 30-90μg/mL with correlation coefficient values 0.9999. The mean recoveries were found in the range of 99.73-100.84% and the %RSD was found to be less than 2% indicated, that the method is more accurate and precise. The Limit of Detection and Limit of Quantification were 0.11μg/ml and 0.32ì g/ml respectively, which indicate sensitivity of the method. No interfering peaks were found in the chromatogram indicating that excipients used in tablet formulations did not interfere with the estimation of the drug by proposed HPLC method.

Keywords

Ormeloxifene, RP-HPLC.

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A. Suneetha ¹, T. Chandana Priya ¹

Affiliations
1 Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur - 522 002, A. P., IN

Abstract

A RP-HPLC method is developed for simultaneous estimation of paracetamol and ondansetron in bulk and oral suspension. The mobile phase used was a combination of potassium dihydrogen orthophosphate buffer: acetonitrile (adjusted to pH 3.0 using orthophosphoric acid) 60:40 v/v. The reversed phase column used was Thermo Hypersil BDS C8 (250 × 4.6 mm i.d., 5μm) at ambient temperature. The detection of the combined dosage form was carried out at 258 nm and a flow rate employed was 1.2 ml/min. The retention time for paracetamol and ondansetron was found to be 3.23min and 4.48min respectively. The method was validated in terms of linearity, range, accuracy, precision, limit of detection (LOD) and limit of quantitation (LOQ). The linearity for paracetamol and ondansetron was found in the concentration range of 31.25- 187.5 μg/ml and 0.5 - 3.0 μg/ml respectively. The percentage recoveries of paracetamol and ondansetron were found to be 100.29 and 100.39 respectively. The percentage relative standard deviation (% RSD) of paracetamol and ondansetron for intraday precision were found to be 0.165 and 0.169 respectively. The LOD and LOQ values for paracetamol and ondansetron were calculated. Thus the proposed method was successfully applied for simultaneous estimation of paracetamol and ondansetron for routine analysis.

Keywords

Paracetamol, Ondansetron and RP-HPLC.

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S. Kathirvel ¹, R. Raju ¹, B. Seethadevi ², A. Suneetha ³, K. Jyothisree ³

Affiliations
1 Department of Pharmaceutical Analysis, National College of Pharmacy, Manassery, Kozhikode, Kerala, 673602, IN
2 Department of Pharmacognosy, National College of Pharmacy, Manassery, Kozhikode, Kerala, 673602, IN
3 Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi road, Guntur, A.P, 522002, IN

Abstract

The study is a proposition of the application of high performance liquid chromatography (HPLC) with a spectrophotometric UV range detector to analyse the chemical purity and assay of tetrabenazine in bulk drug and tablet formulation and also to apply the proposed method to determine the content uniformity according to USP specification. During computer assisted literature search only a few publications were found about tetrabenazine, so the ultimate objective of the present investigation was aimed to develop stability - indicating LC method and the development of the newer analytical method was achieved under optimised chromatographic conditions by using mobile phase composed of potassium phosphate buffer: acetonitrile in the ratio (40:60v /v). The seperation was achieved by using isocratic elution mode with a flow rate of 1.0 mL/min. The effluent was monitored on a UV detector at 284 nm. The retention time of tetrabenazine was found to be at 5.05min and the standard calibration curve was linear over a concentration range of 6.25-37.5 μg/mL with r value of 0.999. The LOD and LOQ were found to be 0.562 and 1.704 μg/mL respectively. The recovery studies were performed and the percentage recoveries were found to be in the range of 98.64-98.88 %. The drug was also subjected to acidic, basic, oxidative and photolytic degradation as per the ICH guidelines. The developed method allows to separate all degradation from tetrabenazine and to quantitate the tetrabenazine amount. As there is no official monograph in the pharmacopeias about tetrabenazine, the performed full validation procedure makes the method ready to use in routine analysis.

Keywords

Content Uniformity, ICH Guidelines, Tetrabenazine And USP Specification.

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A. Suneetha ¹, K. Manasa ¹

Affiliations
1 Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur, A.P - 522002, IN

Abstract

A simple, sensitive, precise and accurate UV Spectrophotometric method has been developed for estimation of andrographolide in kalmegh plant extract. The method is based on the formation of red-orange colored complex with picric acid in an alkaline medium and obeys beer's law in the range of 10 - 100μg/mL exhibiting the maximum absorbance at 481nm. The limit of detection and quantification were found to be 1.0μg/mL and 3.0μg/mL, respectively. The calibration curve demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient 0.9998. The Regression equation of the curve was Y=0.0114x + 0.0067. The method was validated according to ICH guidelines.

Keywords

Andrographolide, Picric Acid, NaOH, Validation, ICH Guidelines.

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S. Kathirvel ¹, A. Suneetha ²

Affiliations
1 Hindu College of Pharmacy, Amaravathi Road, Guntur-500 002, A.P, IN
2 Hindu College of Pharmacy, Amaravathi Road, Guntur, Andhra Pradesh-522002, IN

Abstract

A simple, rapid, sensitive and precise HPLC method has been developed for the estimation of risperidone in oral solution. In this method RP-C18 Phenominex Gemini column (250 mm x 4.6 mm i.d.,5μm particle size) with mobile phase consisting of 0.5% ammonium acetate buffer, acetonitrile and methanol in the ratio of 50:20:30 v/v/v in isocratic mode was used. The detection wavelength is 260 nm and the flow rate is 1.5 mL/min. In the range of 20-100μg/mL, the linearity of risperidone shows a correlation coefficient of 0.9999. The percentage recovery ranges from 98.95-101.50%. The proposed method was validated by determining sensitivity, accuracy, precision and linearity.

Keywords

Risperidone, HPLC, Oral Solution, Validation.

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A. Suneetha ¹, B. Syama Sundar ²

Affiliations
1 Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur (A.P), IN
2 Department of Pharmacy, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur (A.P), IN

Abstract

A simple, sensitive, precise, accurate and economical spectrophotometric method of analysis for escitalopram oxalate both as a bulk drug and in tablet dosage forms was developed and validated. The method employed methanol as solvent and the drug shows maximum absorbance at 239 nm with molar absorptivity of 0.1742×10⁵ L/mol.cm. The linear regression analysis data for the calibration plot showed good linear relationship with r² = 0.9999 in the concentration range of 5-25 μg/ml. Results of analysis were validated statistically and by recovery studies.

Keywords

Escitalopram Oxalate, U.V Spectrophotometry, Tablets.

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S. Kathirvel ¹, R. Raju ¹, B. Seethadevi ¹, A. Suneetha ², J. Pavani ²

Affiliations
1 Department of Pharmaceutical Analysis, National College of Pharmacy, Manassery, Mukkom Post, Kerala, IN
2 Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur, A.P, IN

Abstract

The objective of the current study was to develop a validated, specific and stability-indicating reversed phase HPLC method for the quantitative determination of posaconazole and its related substances in API (Active Pharmaceutical Ingredient). The determination was done for active pharmaceutical ingredient in the presence of degradation products, and its process-related impurities. The chromatographic separation was achieved on a waters HPLC system with PDA detector and the column employed for the present investigation was inertsil ODS-3V C18 (150×4.6 mm with 5 μ particle size) and empower2 software provided by waters was used throughout the experiment. The method employed a linear gradient elution and the detection wavelength was set at 225 nm (for intermediate A impurity) and 260 nm (for intermediate B, diastereomer, formyl and benzyl posaconazole impurity). The drug was subjected to stress conditions of hydrolysis (acid and base), oxidation, photolysis and thermal degradation as per International Conference on Harmonization (ICH) prescribed stress conditions to show the stability-indicating power of the method. Significant degradation was observed during acid, oxidative, thermal and photo stress studies. In the developed HPLC method, the resolution between posaconazole and its process-related impurities was found to be greater than 2.0. Regression analysis shows an r value (correlation coefficient) of greater than 0.999 for posaconazole and it’s all the five impurities. The developed HPLC method was validated with respect to linearity, accuracy, precision and robustness.

Keywords

Degradation Products, Posaconazole, Process-Related Impurities, Regression Analysis.

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A. Suneetha ¹, B. Syama Sundar ²

Affiliations
1 Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur-522002, IN
2 Department of Chemistry/Pharmacy, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur-522510, IN

Abstract

A new simple, sensitive, precise, accurate and economical UV spectrophotometric method of analysis for almotriptan malate both as a bulk drug and in tablet formulations was developed and validated. The method employed methanol as solvent and the drug shows maximum absorbance at 227 nm with molar absorptivity of 0.8532×10⁵ L/mol.cm. The linear regression analysis data for the calibration plot showed good linear relationship with r²=0.9999 in the concentration range of 1-5 μg/ml. Results of analysis were validated statistically and by recovery studies.

Keywords

Almotriptan Malate, U.V Spectrophotometry, Tablets.

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Savithri Pasupuleti ¹, Nirmala Jyothi Bollineni ¹, A. Suneetha ¹

Affiliations
1 Department of Community Health Nursing, NRI College of Nursing, Chinakakani, Guntur, AP, IN

Abstract

Nutrition is a basic human need and a prerequisite to a healthy life. In India about 35 percent of total population consists of children below 15 years of age. Malnutrition is the most wide spread condition affecting health of the children, there is an urgent need to institute supplementary feeding programmes in the developing countries. Hence the investigator had undertaken this study to evaluate the effectiveness of Krishna Poshak Mix on nutritional status of rural anganawadi children of Pedaparimi village, Mangalgiri Mandalam, Guntur district, Andhra Pradesh.” Methodology: A true- experimental, pre-test post-test control group research approach was adopted as research design in this study. Fifty subjects were selected by using simple random sampling technique who were assigned into Experimental group (n=25) and control group (n=25). Findings: The results of the study revealed that Krishna Poshak mix laddus were effective in improving the nutritional status of the preschool children. As it was evidenced by their improved mean values form the initial assessment of weight (12.72) and UMAC (13.16) in experimental group and in control group weight (12.01) and UMAC (12.91) to after 6 weeks assessment of weight (13.31) with ‘t’ value (29) and UMAC (13.6) with ‘t’ value (17.4) in experimental group and the control group mean values of weight (12.04) with ‘t’ value (0.54) and UMAC (12.91) with t’ value (1) which shows statistically significance association at p<0.05 level. Conclusion: The overall study findings concluded that KPM laddus as supplementary nutrition is an effective strategy which can help in improving anthropometric measurements of children and this can indirectly help to reduce the mortality and morbidity rate among the preschool children by recuperating the immune levels.

Keywords

KPM, Nutritional Status, Anganawadi Children.

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Md. Abdul Sattar ¹, A. Suneetha ¹

Affiliations
1 Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur-522002, Andhra Pradesh, IN

Abstract

Numerous analysts have endeavored to utilize computer supported structure (Computer assisted design) or CAD and computer helped fabricating (CAM) to know a framework that provides a three-dimensional (3D) condition for recovery of tissues and organs. Therefore, a number of 3D printing advances, as well as stereo lithography, testimony displaying, inkjet-based printing and specific optical maser sintering are created. Since these 3D printing advancements use PCs for structure and manufacture, and that they will produce 3D platforms as structured; as a result, they will be institutionalized. Development of target tissues and organs needs the distance of fitting development factors; therefore manufacture of 3D platform frameworks that discharge these bimolecular has been investigated. The medication was created utilizing Pennsylvania-based Aprecia Pharmaceutical's exclusive ZipDose innovation,that expands once existing 3DP analysis did by MIT into creating fast dissolving materials. Even so empowering the generation of more and more compelling portion organizes; the innovation is at risk of have a key influence within the pharmaceutical business' flip towards low-volume generation and customized prescription. Going higher than and on the far side, on-request medicates printing offices at centers and drug stores, or maybe in patients' homes, may allow specialists to reinforce treatment by creating made-to-order dosing regimens. What are additional, parts might be made-to-order with singular hues, flavors and shapes to talk to person understanding which can support their adherence. As 3D printing capacities grow any, security and body issues ar attended and therefore the expense of the innovation falls besides contract manufacturers and pharmaceutical organizations that explore totally different avenues relating to these 3D printing developments are likely to choose up an aggressive edge. A new method was established for simultaneous estimation of Velpatasvir and Voxilaprevir by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Velpatasvir and Voxilaprevir by using Thermosil C18 column (4.0×125mm) 5.0μm, flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) methanol: Sodium acetate buffer pH 3 (pH was adjusted with orthophosphoricacid), detection wavelength was 252nm. The retention times were found to be 2.566 mins and 3.417 mins. The linearity study of Velpatasvir and Voxilaprevir was found in concentration range of 5μg-25μg and 50μg-250μg and correlation coefficient (r²) was found to be 0.999 and 0.999, % recovery was found to be 99.56% and 99.48%, %RSD for repeatability was 0.86 and 0.82, % RSD for intermediate precision was 0.44 and 0.19 respectively. The precision study was precise, robust, and repeatable.LOD value was 3.17 and 5.68, and LOQ value was 0.0172 and 0.2125 respectively. Hence the suggested RP-HPLC

Keywords

Thermosil C18 column, Velpatasvir and Voxilaprevir , RP-HPLC.

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Chimata Venkateswarlu ¹, B. Nirmala Jyothi ², A. Suneetha ²

Affiliations
1 M.Sc. Nursing, Department of Community Health Nursing, NRI College of Nursing, Chinakakani, Guntur (Dt), Andhra Pradesh., IN
2 Professor, Department of Community Health Nursing, NRI College of Nursing, Chinakakani, Guntur (Dt), Andhra Pradesh., IN

Abstract

Background: Attention-Deficit Hyperactivity Disorder (ADHD) is one of the popular neuro developmental disorder among kids, adolescents and in adults. The main Objective of the study isto assess the effectiveness of a video awareness programme regarding Attention deficit hyperactivity disorder (ADHD) among primary school teachers and parents of primary school children.Materials and Methods: A pre experimental, one group pre-test and post-test research design was adopted for this study. The study was conducted in selected schools of Mangalagiri, Guntur district, Andhra Pradesh. Fifty subjects of primary school teachers and sixty subjects of parents were selected by using convenient sampling technique. A structured questionnaire was developed to collect the sociodemographic data and Knowledge on Attention Deficit Disorders scale (KADDS) was used to assess the effectiveness of Video awareness programme regarding ADHD. Results: The findings of the study shown that Majority (62%) of the primary school teachers had bachelor’s degree in elementary education. Where as in parents’group majority (53%) of the parents had high school certificate. In primary school teachers, the mean score of pre-test was 16.78±3.78, and post-test mean score was 31.5±2.74, the obtained ‘t’ value (t=20.84, df = 49). In parents of primary school children group,the pre-test mean score was 15.06±3.91 and post-test mean score was 28.11±4.35, the obtained ‘t’ value (t=17.64, df= 59). The chi-square shown that there was a significant association between the primary school teachers pre-test level of knowledge with their (χ2=6.04) age, (χ2=8.7) education level,in parents of primary school children, there was a significant association between pre-test level of knowledge with their education level (χ2=19.64), family income (χ2=5.49). Conclusion:The study concluded that the level of knowledge was improved after administration of Video. Thus, Video is an effective strategy which can help in improving the level of knowledge among primary school teachers and parents of primary school children.

Keywords

Attention Deficit Hyperactivity Disorder, Primary School Teachers, Parents, Knowledge, Video Awareness Programme.

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